IN OUR LAB SITES YOU WILL FIND
WORLD CLASS LAB EQUIPMENT

PCR
PCR Thermocycler Blue-Ray biotech
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Temperature range +4 °C-99 °C ; Fast heating ramp rate up to 5.5 °C/sec ; Excellent temperature accuracy and uniformity (+/- 0.3 °C) ; 12-section gradient temperature range from 1 to 24.9 °C for PCR optimization ; The quick boot-up takes only 45 seconds.
Temperature range -3°C-99 °C temperature accuracy and uniformity (+/- 0.1 °C)

RT-PCR
Analysis methods: PCR quantification, Gene expression, Melt curve analysis, Allelic discrimination, End point analysis ; Temperature range 0-100°C +/-0.2°C, Range of ext./em. 450-730nm ; 96 wells: from1μL-50 μL (recommended 10μL-25 μL).
Mx3000p QPCR System Agilent
Mx3000p QPCR System Agilent - Analysis methods: Quantitative PCR, Comparative Quantitation, SYBR Green, Allele Discrimination, Melting curve. 4 dyes per well

Microscope
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Olympus Fluorescence Microscope inverted CKX53 Olympus x4, x10, x20 + Fluorescence GFP, RFP, DAPI Brightfield, Phase Contrast, and DIC only
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Motic Optical Microscope x4, x10, x40, x100 + Camera 5M
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Olympus Binocular Microscope up to x90 + Camera 15M

Plate Reader
Synergy HT Multi-Mode Microplate Reader- Capable of reading any plate format up to 384-well plates and provides temperature control up to 50° C and shaking as standard features. Typical applications include antibody-antigen binding, receptor-liquid binding, ELISA, nucleic acid quantitation
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Absorbance monochromator from 200 nm to 999 nm (UV -Vis).
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Fluorescence (ext./em. 485/530, 528/590, 485/590).
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Luminescence.
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Thermo scientific Multiskan FC- Plate types – 96 or 348 well plate ; Wavelength – 405nm, 450nm, 620nm ; Shaker – Slow (5 Hz, amplitude 15 mm), Medium (11 Hz, amplitude 3 mm), and Fast (20 Hz, amplitude 1 mm) ; Temperature range - +4°C to 50°C.
PHOmo Microplate reader- Detection method: Absorbance ; Measurement modes: Endpoint & Kinetic ; Plate type: 96, 48 flat, U and V bottom ; Filters 405nm, 450nm, 492nm, 620nm.

Flow cytometry - FACS
CytoFLEX Beckman Coulter Life Science Flow cytometr
Laser specification- Violet (ex.405nm), Blue (ex.488), Red (ex.638). 30 000 events per second with all configured parameters.

Nano Drop
Micro-Spectrophotometer Fluorometer DeNovix DS-11 FX
Rapidly quantify seven orders of magnitude of nucleic acids and protein samples selecting your choice of UV-Vis or Fluorescent modes.
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Full spectrum UV-Vis analysis (190nm to 840 nm)
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Fluorescence capability:
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Green LED : 525 nm (Excitation), 565 to 650 nm (Emission)
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Red LED : 632 nm (Excitation), 665 to 740 nm (Emission)
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Blue LED : 470 nm (Excitation), 514 to 567 nm (Emission)
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UV : 375 nm (Excitation), 435 to 485 nm (Emission)
NanoDrop 2000 Thermo Scientific
Applications: Nucleic acid, Protein, Fluorescent dye corporation, BCA Assays, Bradford, Kinetic methods, Custom methods ; Full spectral output (190 - 840 nm) ; Preconfigured methods for A260, A280, labeled DNA or Proteins, and much more.

Gel Imaging system
Semi-Quantitative Assay EtBr, SYBR Green/ safe, Commassie,
Photography of DNA, RNA and protein Gels, Western blot membrane, Dot blot,
Vis; UV (302, 365nm); Chemiluminescence
AmershamTM Imager 600
Chemiluminescent Western blotting detection

Cell counter
Countess™ II Automated Cell Counter
is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells.
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Accurate
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Fast
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Convenient

Centrifuges
Capacity-500/250ml bottle, 50/15ml tube; Maximum speed: 15 200 rpm (depending on the rotor), 4 816xg; Temperature range: -10°C – 40°C
FRESCO 17
Capacity-24 Eppendorf tube ; Maximum speed- 13 000 rpm depending on rotor, 17 000xg ; Temperature range- 5°C – 40°C
MEGAFUGE 16R
Capacity- 4x50ml/ 12x15ml ; Maximum speed Depending on Rotor – 15 200 rpm; 25 830xg ; Temperature range- -10°C – 40°C
NUVE NF 400R
Capacity: 4x50ml/ 8x15ml ; Maximum speed 4 100 rpm; Maximum RCF- 2 819xg ; Temperature range: -9°C – 40°C
NUVE NF 800R
Capacity: 4x50ml/ 12x15ml/ 24x1.5ml or 2ml/ 2 plates ; Maximum speed Swing-out Rotor: 4 100 rpm; Maximum RCF- 3 045xg ; Maximum speed Angle Rotor: 14 000 rpm; Maximum RCF: 18 405xg ; Temperature range: -9°C – 40°C

DNA & Protein electrophoresis Transblot
Detection & Analysis
Protein or DNA electrophoresis
Transblot SD semi dry
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Biorad- Transferring to a membrane by semi dry transfer system.
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Analysis by Commassie staining, immunoblotting: antibodies staining, and Imaging system.

PCR
PCR Thermocycler Blue-Ray biotech
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Temperature range +4 °C-99 °C ; Fast heating ramp rate up to 5.5 °C/sec ; Excellent temperature accuracy and uniformity (+/- 0.3 °C) ; 12-section gradient temperature range from 1 to 24.9 °C for PCR optimization ; The quick boot-up takes only 45 seconds.
Temperature range -3°C-99 °C temperature accuracy and uniformity (+/- 0.1 °C)
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Fusion FX 6 EDGE - Gel Imaging system
Chemiluminescence Western, Northern or Southern blot
Optional applications:
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DNA & RNA gels and fluorescence stain imaging with UV-Pad or Blue-Pad
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Colorimetric stained protein gels, X-Ray film, autorads,
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SSCP gels, colony dish and flask imaging with White-Light-Pad or UV-Pad + conversion screen
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Fluorescence Western blot with Spectra Capsules
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BioTek Synergy H1 Multimode Reader
Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection.
Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.
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UV-Vis Absorbance
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Fluorescence Intensity
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Luminescence
